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The BLOS1-interacting protein KXD1 is involved in the biogenesis of lysosome-related organelles.

ABSTRACT: Biogenesis of lysosome-related organelles (LROs) complex-1 (BLOC-1) is an eight-subunit complex involved in lysosomal trafficking. Interacting proteins of these subunits expand the understanding of its biological functions. With the implementation of the naïve Bayesian analysis, we found that a human uncharacterized 20 kDa coiled-coil KxDL protein, KXD1, is a BLOS1-interacting protein. In vitro binding assays confirmed the interaction between BLOS1 and KXD1. The mouse KXD1 homolog was widely expressed and absent in Kxd1 knockout (KO) mice. BLOS1 was apparently reduced in Kxd1-KO mice. Mild defects in the melanosomes of the retinal pigment epithelia and in the platelet dense granules of the Kxd1-KO mouse were observed, mimicking a mouse model of mild Hermansky-Pudlak syndrome that affects the biogenesis of LROs.

SUBMITTER: Yang Q

PROVIDER: S-EPMC3800082 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature

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The BLOS1-interacting protein KXD1 is involved in the biogenesis of lysosome-related organelles.

Yang Qing Q He Xin X Yang Lin L Zhou Zhiyong Z Cullinane Andrew R AR Wei Aihua A Zhang Zhe Z Hao Zhenhua Z Zhang Aili A He Min M Feng Yaqin Y Gao Xiang X Gahl William A WA Huizing Marjan M Li Wei W

Traffic (Copenhagen, Denmark) 20120528 8

Biogenesis of lysosome-related organelles (LROs) complex-1 (BLOC-1) is an eight-subunit complex involved in lysosomal trafficking. Interacting proteins of these subunits expand the understanding of its biological functions. With the implementation of the naïve Bayesian analysis, we found that a human uncharacterized 20 kDa coiled-coil KxDL protein, KXD1, is a BLOS1-interacting protein. In vitro binding assays confirmed the interaction between BLOS1 and KXD1. The mouse KXD1 homolog was widely exp ...[more]

PMID: 22554196

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