Unknown

Dataset Information

0

Mutations in HIV-1 reverse transcriptase cause misfolding and miscleavage by the viral protease.


ABSTRACT: Previous work on mutations in the thumb of HIV-1 reverse transcriptase (RT) showed that the majority of the mutant RTs were degraded (by the viral protease) to various extents in virions. This degradation was, in most cases, temperature sensitive, and presumably was due to a partial unfolding of the protein at 37°C. We used recombinant proteins to investigate the effects of the mutations on the thermal stability and proteolytic degradation of RT. Both subunits contribute to the stability of RT. In general, the differences in stability between the mutants and WT were greater if the mutation was in p51 rather than p66. Expressing the Pol polyprotein containing the RT mutants in Escherichia coli produced results similar to what was seen in virions; the mutant RTs were misfolded and/or degraded at 37°C, but were better folded and processed at 30°C.

SUBMITTER: Dunn LL 

PROVIDER: S-EPMC3804327 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

Mutations in HIV-1 reverse transcriptase cause misfolding and miscleavage by the viral protease.

Dunn Linda L LL   Boyer Paul L PL   Clark Patrick K PK   Hughes Stephen H SH  

Virology 20130711 1-2


Previous work on mutations in the thumb of HIV-1 reverse transcriptase (RT) showed that the majority of the mutant RTs were degraded (by the viral protease) to various extents in virions. This degradation was, in most cases, temperature sensitive, and presumably was due to a partial unfolding of the protein at 37°C. We used recombinant proteins to investigate the effects of the mutations on the thermal stability and proteolytic degradation of RT. Both subunits contribute to the stability of RT.  ...[more]

Similar Datasets

| S-EPMC2696282 | biostudies-literature
| S-EPMC4907232 | biostudies-literature
| S-EPMC8387031 | biostudies-literature
2023-11-30 | E-MTAB-13563 | biostudies-arrayexpress
2019-07-29 | E-MTAB-8153 | biostudies-arrayexpress
| S-EPMC7051075 | biostudies-literature
| S-EPMC2573056 | biostudies-literature
| S-EPMC1866358 | biostudies-literature
| S-EPMC4302432 | biostudies-literature
| S-EPMC4054409 | biostudies-literature