Ontology highlight
ABSTRACT:
SUBMITTER: Sczepanski JT
PROVIDER: S-EPMC3804424 | biostudies-literature | 2013 Sep
REPOSITORIES: biostudies-literature
Journal of the American Chemical Society 20130828 36
An L-RNA aptamer was developed that binds the natural D-form of the HIV-1 trans-activation responsive (TAR) RNA. The aptamer initially was obtained as a D-aptamer against L-TAR RNA through in vitro selection. Then the corresponding L-aptamer was prepared by chemical synthesis and used to bind the desired target. The L-aptamer binds D-TAR RNA with a Kd of 100 nM. It binds D-TAR exclusively at the six-nucleotide distal loop, but does so through tertiary interactions rather than simple Watson-Crick ...[more]