Evolution and characterization of a benzylguanine-binding RNA aptamer.
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ABSTRACT:
SUBMITTER: Xu J
PROVIDER: S-EPMC5020705 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
Publications
Evolution and characterization of a benzylguanine-binding RNA aptamer.
Xu J J Carrocci T J TJ Hoskins A A AA
Chemical communications (Cambridge, England) 20151105 3
Repurposing the "protein-labeling toolkit" for RNA research could be a pragmatic approach for developing new RNA-labeling methods. We have evolved an RNA aptamer that tightly binds benzylguanine (bG), the key ligand for the protein SNAP-tag. The aptamer tightly binds bG fluorophores and can be purified from cellular RNA with bG agarose under native conditions. ...[more]
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