Ontology highlight
ABSTRACT: Background
Tuberculosis is transmitted by patients with pulmonary disease. Matrix metalloproteinases (MMPs) drive lung destruction in tuberculosis but the resulting matrix degradation products (MDPs) have not been studied. We investigate the hypothesis that MMP activity generates matrix turnover products as correlates of lung pathology.Methods
Induced sputum and plasma were collected prospectively from human immunodeficiency virus (HIV) positive and negative patients with pulmonary tuberculosis and controls. Concentrations of MDPs and MMPs were analyzed by ELISA and Luminex array in 2 patient cohorts.Results
Procollagen III N-terminal propeptide (PIIINP) was 3.8-fold higher in induced sputum of HIV-uninfected tuberculosis patients compared to controls and desmosine, released during elastin degradation, was 2.4-fold higher. PIIINP was elevated in plasma of tuberculosis patients. Plasma PIIINP correlated with induced sputum MMP-1 concentrations and radiological scores, demonstrating that circulating MDPs reflect lung destruction. In a second patient cohort of mixed HIV seroprevalence, plasma PIIINP concentration was increased 3.0-fold above controls (P < .001). Plasma matrix metalloproteinase-8 concentrations were also higher in tuberculosis patients (P = .001). Receiver operating characteristic analysis utilizing these 2 variables demonstrated an area under the curve of 0.832 (P < .001).Conclusions
In pulmonary tuberculosis, MMP-driven immunopathology generates matrix degradation products.
SUBMITTER: Seddon J
PROVIDER: S-EPMC3805234 | biostudies-literature | 2013 Nov
REPOSITORIES: biostudies-literature
Seddon Jo J Kasprowicz Victoria V Walker Naomi F NF Yuen Ho Ming HM Sunpath Henry H Tezera Liku L Meintjes Graeme G Wilkinson Robert J RJ Bishai William R WR Friedland Jon S JS Elkington Paul T PT
The Journal of infectious diseases 20130806 10
<h4>Background</h4>Tuberculosis is transmitted by patients with pulmonary disease. Matrix metalloproteinases (MMPs) drive lung destruction in tuberculosis but the resulting matrix degradation products (MDPs) have not been studied. We investigate the hypothesis that MMP activity generates matrix turnover products as correlates of lung pathology.<h4>Methods</h4>Induced sputum and plasma were collected prospectively from human immunodeficiency virus (HIV) positive and negative patients with pulmona ...[more]