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Single-cell electroporation using a multifunctional pipette.

ABSTRACT: We present here a novel platform combination, using a multifunctional pipette to individually electroporate single-cells and to locally deliver an analyte, while in their culture environment. We demonstrate a method to fabricate low-resistance metallic electrodes into a PDMS pipette, followed by characterization of its effectiveness, benefits and limits in comparison with an external carbon microelectrode.

SUBMITTER: Ainla A 

PROVIDER: S-EPMC3805499 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature

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Single-cell electroporation using a multifunctional pipette.

Ainla Alar A   Xu Shijun S   Sanchez Nicolas N   Jeffries Gavin D M GD   Jesorka Aldo A  

Lab on a chip 20121101 22

We present here a novel platform combination, using a multifunctional pipette to individually electroporate single-cells and to locally deliver an analyte, while in their culture environment. We demonstrate a method to fabricate low-resistance metallic electrodes into a PDMS pipette, followed by characterization of its effectiveness, benefits and limits in comparison with an external carbon microelectrode. ...[more]

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