Ontology highlight
ABSTRACT:
SUBMITTER: Hombauer H
PROVIDER: S-EPMC3806717 | biostudies-literature | 2011 Dec
REPOSITORIES: biostudies-literature
Hombauer Hans H Srivatsan Anjana A Putnam Christopher D CD Kolodner Richard D RD
Science (New York, N.Y.) 20111201 6063
In eukaryotes, it is unknown whether mismatch repair (MMR) is temporally coupled to DNA replication and how strand-specific MMR is directed. We fused Saccharomyces cerevisiae MSH6 with cyclins to restrict the availability of the Msh2-Msh6 mismatch recognition complex to either S phase or G2/M phase of the cell cycle. The Msh6-S cyclin fusion was proficient for suppressing mutations at three loci that replicate at mid-S phase, whereas the Msh6-G2/M cyclin fusion was defective. However, the Msh6-G ...[more]