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Crystal structure of a glucose/H+ symporter and its mechanism of action.


ABSTRACT: Glucose transporters are required to bring glucose into cells, where it is an essential energy source and precursor in protein and lipid synthesis. These transporters are involved in important common diseases such as cancer and diabetes. Here, we report the crystal structure of the Staphylococcus epidermidis glucose/H(+) symporter in an inward-facing conformation at 3.2-Å resolution. The Staphylococcus epidermidis glucose/H(+) symporter is homologous to human glucose transporters, is very specific and has high avidity for glucose, and is inhibited by the human glucose transport inhibitors cytochalasin B, phloretin, and forskolin. On the basis of the crystal structure in conjunction with mutagenesis and functional studies, we propose a mechanism for glucose/H(+) symport and discuss the symport mechanism versus facilitated diffusion.

SUBMITTER: Iancu CV 

PROVIDER: S-EPMC3816430 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature

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Crystal structure of a glucose/H+ symporter and its mechanism of action.

Iancu Cristina V CV   Zamoon Jamillah J   Woo Sang Bum SB   Aleshin Alexander A   Choe Jun-yong JY  

Proceedings of the National Academy of Sciences of the United States of America 20131014 44


Glucose transporters are required to bring glucose into cells, where it is an essential energy source and precursor in protein and lipid synthesis. These transporters are involved in important common diseases such as cancer and diabetes. Here, we report the crystal structure of the Staphylococcus epidermidis glucose/H(+) symporter in an inward-facing conformation at 3.2-Å resolution. The Staphylococcus epidermidis glucose/H(+) symporter is homologous to human glucose transporters, is very specif  ...[more]

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