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Hydrogel-stabilized droplet bilayers for high speed solution exchange.

ABSTRACT: Many applications utilizing artificial lipid bilayers require the ability to exchange the bilayer's solution environment. However, because of the instability of the bilayer, the rate of solution exchange is limited, which significantly hinders the measurement rate and throughput. We have developed an artificial bilayer system that can withstand high flow speeds, up to 2.1 m/s, by supporting the bilayer with a hydrogel. We demonstrated the ability to measure during flow by measuring the conductance of gramicidin-A channels while switching between solutions of two different compositions, recording a time to measure 90% change in current of approximately 2.7 seconds at a flow rate of 0.1 m/s. We also demonstrated a potential application of this system by measuring the conductance modulation of the rat TRPM8 ion channel by an agonist and antagonist at varying concentrations, obtaining 7-point IC50 and EC50 values in approximately 7 minutes and 4-point values within 4 minutes.

SUBMITTER: Acharya SA

PROVIDER: S-EPMC3817439 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

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Hydrogel-stabilized droplet bilayers for high speed solution exchange.

Acharya Shiv A SA Portman Alexander A Salazar Carl S CS Schmidt Jacob J JJ

Scientific reports 20131105

Many applications utilizing artificial lipid bilayers require the ability to exchange the bilayer's solution environment. However, because of the instability of the bilayer, the rate of solution exchange is limited, which significantly hinders the measurement rate and throughput. We have developed an artificial bilayer system that can withstand high flow speeds, up to 2.1 m/s, by supporting the bilayer with a hydrogel. We demonstrated the ability to measure during flow by measuring the conductan ...[more]

PMID: 24190577

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