Ontology highlight
ABSTRACT:
SUBMITTER: Das U
PROVIDER: S-EPMC3818043 | biostudies-literature | 2013 Nov
REPOSITORIES: biostudies-literature
Das Uddipan U Kumar Nitesh N Gourinath Samudrala S Srinivasan Alagiri A
Acta crystallographica. Section F, Structural biology and crystallization communications 20131017 Pt 11
The Mycobacterium tuberculosis vapBC15 locus encodes a toxin-antitoxin complex. VapC-15 is a toxin and possesses ribonuclease activity and VapB-15 is an antitoxin which both binds and inhibits the VapC-15 toxin. In this study, vapBC15 genes were cloned and co-expressed in Escherichia coli. The complex was purified to homogeneity by affinity and size-exclusion chromatography. The VapBC-15 complex was crystallized using the sitting-drop vapour-diffusion technique. The crystals diffracted to 2.6 Å ...[more]