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The deSUMOylase SENP7 promotes chromatin relaxation for homologous recombination DNA repair.

ABSTRACT: SUMO conjugation is known to occur in response to double-stranded DNA breaks in mammalian cells, but whether SUMO deconjugation has a role remains unclear. Here, we show that the SUMO/Sentrin/Smt3-specific peptidase, SENP7, interacts with the chromatin repressive KRAB-associated protein 1 (KAP1) through heterochromatin protein 1 alpha (HP1?). SENP7 promotes the removal of SUMO2/3 from KAP1 and regulates the interaction of the chromatin remodeler CHD3 with chromatin. Consequently, in the presence of CHD3, SENP7 is required for chromatin relaxation in response to DNA damage, for homologous recombination repair and for cellular resistance to DNA-damaging agents. Thus, deSUMOylation by SENP7 is required to promote a permissive chromatin environment for DNA repair.

SUBMITTER: Garvin AJ

PROVIDER: S-EPMC3818072 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

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The deSUMOylase SENP7 promotes chromatin relaxation for homologous recombination DNA repair.

Garvin Alexander J AJ Densham Ruth M RM Blair-Reid Sarah A SA Pratt Kenny M KM Stone Helen R HR Weekes Daniel D Lawrence Kirsty J KJ Morris Joanna R JR

EMBO reports 20130910 11

SUMO conjugation is known to occur in response to double-stranded DNA breaks in mammalian cells, but whether SUMO deconjugation has a role remains unclear. Here, we show that the SUMO/Sentrin/Smt3-specific peptidase, SENP7, interacts with the chromatin repressive KRAB-associated protein 1 (KAP1) through heterochromatin protein 1 alpha (HP1α). SENP7 promotes the removal of SUMO2/3 from KAP1 and regulates the interaction of the chromatin remodeler CHD3 with chromatin. Consequently, in the presence ...[more]

PMID: 24018422

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