Dataset Information

Fasciola hepatica expresses multiple alpha- and beta-tubulin isotypes.

ABSTRACT: We have identified five alpha-tubulin and six beta-tubulin isotypes that are expressed in adult Fasciola hepatica. Amino acid sequence identities ranged between 72 and 95% for fluke alpha-tubulin and between 65 and 97% for beta-tubulin isotypes. Nucleotide sequence identity ranged between 68-77% and 62-80%, respectively, for their coding sequences. Phylogenetic analysis indicated that two of the alpha-tubulins and two of the beta-tubulins were distinctly divergent from the other trematode and nematode tubulin sequences described in this study, whereas the other isotypes segregated within the trematode clades. With regard to the proposed benzimidazole binding site on beta-tubulin, three of the fluke isotypes had tyrosine at position 200 of beta-tubulin, two had phenylalanine and one had leucine. All had phenylalanine at position 167 and glutamic acid at position 198. When isotype RT-PCR fragment sequences were compared between six individual flukes from the susceptible Cullompton isolate and from seven individual flukes from the two resistant isolates, Sligo and Oberon, these residues were conserved.

SUBMITTER: Ryan LA

PROVIDER: S-EPMC3820024 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature

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Fasciola hepatica expresses multiple alpha- and beta-tubulin isotypes.

Ryan Louise A LA Hoey Elizabeth E Trudgett Alan A Fairweather Ian I Fuchs Marc M Robinson Mark W MW Chambers Emma E Timson David J DJ Ryan Eimear E Feltwell Theresa T Ivens Al A Bentley Geoffrey G Johnston David D

Molecular and biochemical parasitology 20080214 1

We have identified five alpha-tubulin and six beta-tubulin isotypes that are expressed in adult Fasciola hepatica. Amino acid sequence identities ranged between 72 and 95% for fluke alpha-tubulin and between 65 and 97% for beta-tubulin isotypes. Nucleotide sequence identity ranged between 68-77% and 62-80%, respectively, for their coding sequences. Phylogenetic analysis indicated that two of the alpha-tubulins and two of the beta-tubulins were distinctly divergent from the other trematode and ne ...[more]

PMID: 18372053

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Project description:BackgroundFasciola hepatica (liver fluke) affects grazing animals including horses but the extent to which it affects UK horses is unknown.ObjectivesTo define how liver fluke affects the UK horse population.Study designDescriptive, cross-sectional, observational study.MethodsAn F. hepatica excretory-secretory antibody detection ELISA with a diagnostic sensitivity of 71% and specificity of 97% was validated and used to analyse serum samples. An abattoir study was performed to determine prevalence. A case-control study of 269 horses compared fluke exposure between horses with liver disease and controls. Data on clinical signs and blood test results were collected for sero-positive horses. Genotyping of adult fluke was used to produce a multilocus genotype for each parasite.ResultsFour (2.2%) of 183 horses registered in the UK, sampled in the abattoir, had adult flukes in the liver, and the sero-prevalence of F. hepatica was estimated as 8.7%. In the case-control study, horses showing signs consistent with liver disease had significantly higher odds of testing positive for F. hepatica on ELISA than control horses. In 23 sero-positive horses, a range of non-specific clinical signs and blood test abnormalities was reported, with a third of the horses showing no signs. Genotypic analysis of liver flukes from horses provided evidence that these came from the same population as flukes from sheep and cattle.Main limitationsBias could have arisen in the prevalence and case-control studies due to convenience sampling methods, in particular the geographic origin of the horses. Only a small number of horses tested positive so the data on clinical signs are limited.ConclusionsExposure to liver fluke occurs frequently in horses and may be an under-recognised cause of liver disease. Flukes isolated from horses are from the same population as those found in ruminants. When designing and implementing parasite control plans, fluke should be considered, and horses should be tested if appropriate.

Dataset Information

Fasciola hepatica expresses multiple alpha- and beta-tubulin isotypes.

Publications

Fasciola hepatica expresses multiple alpha- and beta-tubulin isotypes.

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