Fasciola hepatica
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA12611 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| CCMX01.dat.gz | Other | |||
| CCMX01.fasta.gz | Fasta.gz | |||
| CCMX01.master.dat | Other | |||
| CDMT01.dat.gz | Other |
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Similar Datasets
Project description:Fasciola hepatica, commonly known as liver fluke, is a trematode which causes Fasciolosis in ruminants and humans. The outer tegumental coat of F. hepatica is a complex metabolically active biological matrix that is continually exposed to the host immune system. It is highly glycosylated and parasite-derived immunogenic oligosaccharide motifs and glycoproteins are currently being investigated as novel vaccine candidates. We selectively enriched for FhTeg mannosylated glycoprotein subsets using lectin affinity chromatography and identified 369 proteins by mass spectrometric analysis.
2016-08-03 | PXD003911 | Pride
Project description:Liver fluke (Fasciola hepatica) infection is both a welfare and productivity issue in sheep farming. Death can result from acute infection, and deaths are becoming more frequent as anthelmintic resistance increases. New control strategies are desirable, and vaccination is a good option. Previous studies have shown an experimental vaccine based on a F. hepatica protein, cathepsin L1, (rmFhCL1) may be a viable aid to control liver fluke in cattle/sheep, however efficacy is variable. A trial was conducted on sheep immunized with rmFhCL1 following infection with F. hepatica to understand the immune response changes induced by vaccinination at a molecular level . Peripheral blood mononuclear cells (PBMCs) were isolated at four different time points for RNA-Seq analysis. Genes differentially expressed between vaccinated and control animals were identified. Their functional roles were studied using in silico methods.
2016-11-04 | GSE71431 | GEO
Project description:Fasciola hepatica (liver fluke) Transcriptome Assembly
| PRJNA330752 | ENA
Project description:RNAseq analysis of the liver fluke Fasciola hepatica
| PRJEB6904 | ENA
Project description:Purpose: The miRnome of the liver fluke, Fasciola hepatica, has historically been assembled with limited omic's resources using specific life stages. The goals of this study is to quanity the known and published miRNAs and determine novel miRNAs across three intra-mammlian life stages. Methods: Total RNA was extracted from newly ecysted juveniles 24h post excystment, juveniles at 21d post infection in rats and adult worms during hepatic infection in sheep. Total RNA of each sample was prepared for miRNA sequencing library. Sequenced reads were cleaned and quanitified for F. hepatica mature miRNAs from miRBase version 21 and published miRNAs from Ricafrente et al 2021 using Bowtie tool. Cleaned reads were additionally analysed for novel miRNAs using MiRDeep2 tool in conjunction with the F. hepatica genome and mature miRNAs from F. hepatica, S. japonicum, S. mansoni and C.elegans. Known/published and novel miRNA read counts were normalised to CPM. Results: Of all miRNAs known/published and novel, 124 miRNAs now make up the F. hepatica miRnome. Each life stage exhibited a unique miRNA profile, from which NEJs were the most differentially expressed compared to juveniles and adult. Conclusions: Our study is the first to comparatively assess the miRNA profiles of the intrammalian life stages of F.hepatica simultaneously, from which the miRnome has now been expanded from 77 to 124.
2022-05-31 | GSE186948 | GEO
Project description:The liver fluke Fasciola hepatica is an economically important pathogen of livestock. Fasciolosis in humans is an important re-emerging zoonosis, with 180 million people at risk. Development of novel control strategies requires an understanding of parasite virulence and tissue invasion, and of how the parasite evades and modulates the immune response during early infection. A combination of immunological and proteomic analyses was employed to investigate the peritoneal fluid of sheep infected with F. hepatica to characterise early tissue invasion and liver pathogenesis. At 18 days post-infection (dpi), histopathology of sheep liver showed white necrotic foci/tracts indicative of F. hepatica migration. Within the peritoneal fluid of infected animals, specific F. hepatica antigen FhCL1 antibodies coincided with an intense innate and adaptive cellular immune response, with increasing numbers of total leukocytes and a marked eosinophilia (49%). Cytokine qPCR analysis revealed IL-10, IL-12, IL-13, IL-23 and TGFβ were elevated but not statistically significant at 18 dpi compared to uninfected sheep indicating that whilst the immune response is developing it has not polarised to the Th2 type associated with chronic fasciolosis. Proteomic analysis of the peritoneal fluid identified 178 proteins, with 25 proteins more highly expressed in the infected animals, based on fold changes in protein concentration compared to the uninfected animals. Components of the liver extracellular matrix, including collagen, periostin and the adhesion protein, VCAM-1, exhibited increased expression associated with early fasciolosis, which may be important in signalling host immune responses to tissue damage. In early F. hepatica infection cellular infiltration with marked eosinophilia, adaptive immune responses and products of liver pathology are evident in the peritoneum. Although cytokine responses are developing they are mixed and not yet polarised to a Th2-type. We have characterised biomarkers of parasite-induced liver damage which could be exploited for diagnosis, and vaccine development aimed at reducing/preventing liver pathology.
2018-03-26 | PXD005548 | Pride
Project description:some 454 data for the liver fluke Fasciola hepatica, sligo isolate
| PRJEB8349 | ENA
Project description:A draft genome assembly for the digenean liver fluke, Fasciola hepatica
| PRJEB6687 | ENA
Project description:Some RNA-seq reads form different developmental stages of the liver fluke Fasciola hepatica
| PRJEB6948 | ENA
Project description:The liver fluke Fasciola hepatica is a foodborne zoonotic parasite affecting livestock worldwide with increasing relevance in human health. The first developmental stage that the host meets after ingestion of the parasite is the newly excysted juvenile (NEJ), that actively transverse the gut wall and migrates to their final location in the liver. The regulation of the early developmental events in NEJs is still poorly understood and a relevant target for control strategies. Here we investigated the putative involvement of small regulatory RNAs in the invasion process. The small RNA population of the NEJ fall into two classes, one represented by miRNAs and a secondary group of larger (32- 33 nucleotides) tRNA derived sequences. We identified more than 30 different miRNAs most of them belonging to ancient miRNAs conserved in protostomes and metazoans, notably with an miR-125b variant as highly predominant. Remarkably, several protostomian and metazoan conserved families were not detected in consonance with previous reports of drastic miRNome reduction in parasitic flatworms. Additionally, a set of 11 novel miRNAs was identified, probably associated with specific gene regulation expression needs in F. hepatica. While sequence conservation in mature miRNA is high across the metazoan tree, we observed that flatworm miRNAs are more divergent suggesting that mutation rates in parasitic flatworms could be high. Finally, the distinctive presence of tRNA derived sequences, mostly 5' tRNA halves of selected tRNAs in the small RNA population of NEJs could indicate that this parasite uses both miRNA and tRNA fragments for the regulation of gene expression.
2015-10-02 | GSE66490 | GEO