Ontology highlight
ABSTRACT:
SUBMITTER: Dewal MB
PROVIDER: S-EPMC3820426 | biostudies-literature | 2013 Sep
REPOSITORIES: biostudies-literature
Dewal Mahender B MB Firestine Steven M SM
Biochemistry 20130830 37
N(5)-CAIR synthetase, an essential enzyme in microorganisms, converts 5-aminoimidazole ribonucleotide (AIR) and bicarbonate to N(5)-CAIR with the aid of ATP. Previous X-ray crystallographic analyses of Aspergillus clavatus N(5)-CAIR synthetase postulated that R271, H273, and K353 were important for bicarbonate binding and for catalysis. As reported here, site-directed mutagenesis of these residues revealed that R271 and H273 are, indeed, critical for bicarbonate binding and catalysis whereas all ...[more]