Enhanced Y1H assays for Arabidopsis.
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ABSTRACT:
SUBMITTER: Gaudinier A
PROVIDER: S-EPMC3821074 | biostudies-literature | 2011 Oct
REPOSITORIES: biostudies-literature
Publications
Enhanced Y1H assays for Arabidopsis.
Gaudinier Allison A Zhang Lifang L Reece-Hoyes John S JS Taylor-Teeples Mallorie M Pu Li L Liu Zhijie Z Breton Ghislain G Pruneda-Paz Jose L JL Kim Dahae D Kay Steve A SA Walhout Albertha J M AJ Ware Doreen D Brady Siobhan M SM
Nature methods 20111030 12
We present an Arabidopsis thaliana full-length transcription factor resource of 92% of root stele-expressed transcription factors and 74.5% of root-expressed transcription factors. We demonstrate its use with enhanced yeast one-hybrid (eY1H) screening for rapid, systematic mapping of plant transcription factor-promoter interactions. We identified 158 interactions with 13 stele-expressed promoters, many of which occur physically or are regulatory in planta. ...[more]
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