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Reporter Selection for Nanotags in Multiplexed Surface Enhanced Raman Spectroscopy Assays.

ABSTRACT: We report a quantitative evaluation of the choice of reporters for multiplexed surface-enhanced Raman spectroscopy (SERS). An initial library consisted of 15 reporter molecules that included commonly used Raman dyes, thiolated reporters, and other small molecules. We used a correlation matrix to downselect Raman reporters from the library to choose five candidates: 1,2-bis(4-pyridyl)ethylene, 4-mercaptobenzoic acid, 3,5-dichlorobenzenthiol, pentachlorothiophenol, and 5,5'-dithiobis(2-nitrobenzoic acid). We evaluated the ability to distinguish the five SERS reporters in a dipstick immunoassay for the biomarker human IgG. Raman nanotags, or gold nanostars conjugated to the five reporters and anti-human IgG polyclonal antibodies were constructed. A linear discriminant analysis approach was used to evaluate the separation of the nanotag spectra in mixtures of fixed ratios.

SUBMITTER: Sanchez-Purra M

PROVIDER: S-EPMC6173495 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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Reporter Selection for Nanotags in Multiplexed Surface Enhanced Raman Spectroscopy Assays.

Sánchez-Purrà Maria M Roig-Solvas Biel B Rodriguez-Quijada Cristina C Leonardo Brianna M BM Hamad-Schifferli Kimberly K

ACS omega 20180906 9

We report a quantitative evaluation of the choice of reporters for multiplexed surface-enhanced Raman spectroscopy (SERS). An initial library consisted of 15 reporter molecules that included commonly used Raman dyes, thiolated reporters, and other small molecules. We used a correlation matrix to downselect Raman reporters from the library to choose five candidates: 1,2-bis(4-pyridyl)ethylene, 4-mercaptobenzoic acid, 3,5-dichlorobenzenthiol, pentachlorothiophenol, and 5,5'-dithiobis(2-nitrobenzoi ...[more]

PMID: 30320250

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