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Insulin-like growth factor-2 (IGF2) loss of imprinting marks a field defect within human prostates containing cancer.


ABSTRACT: Loss of imprinting (LOI) is an epigenetic alteration involving loss of parental origin-specific expression at normally imprinted genes. A LOI for IGF2, a paracrine growth factor, has been implicated in the development of prostate and other cancers. In the current study, we define IGF2 LOI in histologically normal prostate tissues in relationship to tumor foci and gene expression.Microdissected tumor associated (TA) adjacent (2?mm) and distant (10?mm) tissues surrounding tumor foci were generated. IGF2 imprinting in informative prostate tissue sets was quantitated using a fluorescent primer extension assay and expression analyzed utilizing quantitative PCR. DNA methylation analyses were performed using quantitative pyrosequencing.A marked IGF2 LOI was found in adjacent TA tissues (39?±?3.1%) and did not significantly decrease in tissues distant (38?±?5.3%) from tumor foci (45?±?2.9%; P?=?0.21). IGF2 imprinting correlated with IGF2 expression in TA tissues, but not within the tumor foci. Hypomethylation of the IGF2 DMR0 region correlated with decreased IGF2 expression in tumors (P?

SUBMITTER: Bhusari S 

PROVIDER: S-EPMC3825178 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature

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Insulin-like growth factor-2 (IGF2) loss of imprinting marks a field defect within human prostates containing cancer.

Bhusari Sachin S   Yang Bing B   Kueck Jessica J   Huang Wei W   Jarrard David F DF  

The Prostate 20110322 15


<h4>Background</h4>Loss of imprinting (LOI) is an epigenetic alteration involving loss of parental origin-specific expression at normally imprinted genes. A LOI for IGF2, a paracrine growth factor, has been implicated in the development of prostate and other cancers. In the current study, we define IGF2 LOI in histologically normal prostate tissues in relationship to tumor foci and gene expression.<h4>Methods</h4>Microdissected tumor associated (TA) adjacent (2 mm) and distant (10 mm) tissues su  ...[more]

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