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A 680 kb duplication at the FTO locus in a kindred with obesity and a distinct body fat distribution.

ABSTRACT: Common intronic SNPs in the human fat mass and obesity associated (FTO) gene are strongly associated with body mass index (BMI). In mouse models, inactivation of the Fto gene results in a lean phenotype, whereas overexpression of Fto leads to increased food intake and obesity. The latter finding suggests that copy number variants at the FTO locus might be associated with extremes of adiposity. To address this question, we searched for rare, private or de novo copy number variation in a cohort of 985 obese and 869 lean subjects of European ancestry drawn from the extremes of the BMI distribution, genotyped on Affymetrix 6.0 arrays. A ?680?kb duplication, confirmed by real-time PCR and G-to-FISH analyses, was observed between ?rs11859825 and rs9932411 in a 68-year-old male with severe obesity. The duplicated region on chromosome 16 spans the entire genome-wide association studies risk locus for obesity, and further encompasses RBL2, AKTIP, RPGRIP1L and all but the last exon of the FTO gene. Affected family members exhibit a unique obesity phenotype, characterized by increased fat distribution in the shoulders and neck with a significantly increased neck circumference. This phenotype was accompanied by increased peripheral blood expression of RBL2 with no alteration in expression of FTO or other genes in the region. No other duplications or deletions in this region were identified in the cohort of obese and lean individuals or in a further survey of 4778 individuals, suggesting that large rare copy number variants surrounding the FTO gene are not a frequent cause of obesity.

SUBMITTER: Davies RW

PROVIDER: S-EPMC3831078 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature

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A 680 kb duplication at the FTO locus in a kindred with obesity and a distinct body fat distribution.

Davies Robert W RW Lau Paulina P Naing Thet T Nikpay Majid M Doelle Heather H Harper Mary Ellen ME Dent Robert R McPherson Ruth R

European journal of human genetics : EJHG 20130417 12

Common intronic SNPs in the human fat mass and obesity associated (FTO) gene are strongly associated with body mass index (BMI). In mouse models, inactivation of the Fto gene results in a lean phenotype, whereas overexpression of Fto leads to increased food intake and obesity. The latter finding suggests that copy number variants at the FTO locus might be associated with extremes of adiposity. To address this question, we searched for rare, private or de novo copy number variation in a cohort of ...[more]

PMID: 23591406

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Dataset Information

A 680 kb duplication at the FTO locus in a kindred with obesity and a distinct body fat distribution.

Publications

A 680 kb duplication at the FTO locus in a kindred with obesity and a distinct body fat distribution.

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