Ontology highlight
ABSTRACT:
SUBMITTER: Prokhorova IV
PROVIDER: S-EPMC3831192 | biostudies-literature | 2013 Nov
REPOSITORIES: biostudies-literature
Prokhorova Irina V IV Osterman Ilya A IA Burakovsky Dmitry E DE Serebryakova Marina V MV Galyamina Maria A MA Pobeguts Olga V OV Altukhov Ilya I Kovalchuk Sergey S Alexeev Dmitry G DG Govorun Vadim M VM Bogdanov Alexey A AA Sergiev Petr V PV Dontsova Olga A OA
Scientific reports 20131118
Ribosomes contain a number of modifications in rRNA, the function of which is unclear. Here we show--using proteomic analysis and dual fluorescence reporter in vivo assays--that m(2)G966 and m(5)C967 in 16S rRNA of Escherichia coli ribosomes are necessary for correct attenuation of tryptophan (trp) operon. Expression of trp operon is upregulated in the strain where RsmD and RsmB methyltransferases were deleted, which results in the lack of m(2)G966 and m(5)C967 modifications. The upregulation re ...[more]