Ontology highlight
ABSTRACT:
SUBMITTER: Sabat G
PROVIDER: S-EPMC91908 | biostudies-literature | 2000 Feb
REPOSITORIES: biostudies-literature
Sabat G G Rose P P Hickey W J WJ Harkin J M JM
Applied and environmental microbiology 20000201 2
A set of PCR primers targeting 16S rRNA gene sequences was designed, and PCR parameters were optimized to develop a robust and reliable protocol for selective amplification of Escherichia coli 16S rRNA genes. The method was capable of discriminating E. coli from other enteric bacteria, including its closest relative, Shigella. Selective amplification of E. coli occurred only when the annealing temperature in the PCR was elevated to 72 degrees C, which is 10 degrees C higher than the optimum for ...[more]