Ontology highlight
ABSTRACT:
SUBMITTER: Shi D
PROVIDER: S-EPMC3831942 | biostudies-literature | 2013 Nov
REPOSITORIES: biostudies-literature
Shi Dan D Nannenga Brent L BL Iadanza Matthew G MG Gonen Tamir T
eLife 20131119
We demonstrate that it is feasible to determine high-resolution protein structures by electron crystallography of three-dimensional crystals in an electron cryo-microscope (CryoEM). Lysozyme microcrystals were frozen on an electron microscopy grid, and electron diffraction data collected to 1.7 Å resolution. We developed a data collection protocol to collect a full-tilt series in electron diffraction to atomic resolution. A single tilt series contains up to 90 individual diffraction patterns col ...[more]