Unknown

Dataset Information

0

On-site education of VEGF-recruited monocytes improves their performance as angiogenic and arteriogenic accessory cells.


ABSTRACT: Adult neovascularization relies on the recruitment of monocytes to the target organ or tumor and functioning therein as a paracrine accessory. The exact origins of the recruited monocytes and the mechanisms underlying their plasticity remain unclear. Using a VEGF-based transgenic system in which genetically tagged monocytes are conditionally summoned to the liver as part of a VEGF-initiated angiogenic program, we show that these recruited cells are derived from the abundant pool of circulating Ly6C(hi) monocytes. Remarkably, however, upon arrival at the VEGF-induced organ, but not the naive organ, monocytes undergo multiple phenotypic and functional changes, endowing them with enhanced proangiogenic capabilities and, importantly, with a markedly increased capacity to remodel existing small vessels into larger conduits. Notably, monocytes do not differentiate into long-lived macrophages, but rather appear as transient accessory cells. Results from transfers of presorted subpopulations and a novel tandem transfer strategy ruled out selective recruitment of a dedicated preexisting subpopulation or onsite selection, thereby reinforcing active reprogramming as the underlying mechanism for improved performance. Collectively, this study uncovered a novel function of VEGF, namely, on-site education of recruited "standard" monocytes to become angiogenic and arteriogenic professional cells, a finding that may also lend itself for a better design of angiogenic therapies.

SUBMITTER: Avraham-Davidi I 

PROVIDER: S-EPMC3832929 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC1920536 | biostudies-literature
| S-EPMC4976335 | biostudies-literature
| S-EPMC4401781 | biostudies-literature
| S-EPMC7978382 | biostudies-literature
| S-EPMC3022671 | biostudies-literature
| S-EPMC3055780 | biostudies-literature
| S-EPMC4536358 | biostudies-literature
| S-EPMC3017177 | biostudies-literature
| S-EPMC5501755 | biostudies-literature
| S-EPMC2651676 | biostudies-literature