Ontology highlight
ABSTRACT: Background
CCR5-delta32 heterozygous individuals are susceptible to HIV-1. However, it is not clear if there is a relevant protective effect against transmission and a beneficial effect in terms of HIV progression which cannot be attributed to CCR5 surface density alone. Therefore we investigated HIV-1 dependency factors (HDF) which might be differently regulated in CCR5 wild type (WT) and CCR5-delta32 heterozygous individuals.Methods
We examined CD34+ hematopoietic progenitor cells derived from bone marrow samples from 19 healthy volunteers, 12 individuals with CCR5 WT and 7 with heterozygous CCR5-delta32 deletion. Samples were analyzed using a global gene expression oligonucleotide microarray (HG-U133plus 2.0, Affymetrix Inc.).Results
A total of 205 genes were found with altered expression (3fold difference, present call rate of 75%, p?ConclusionThe CCR5-delta32 deletion is associated with other HDFs in HIV-1 pathogenesis as a possible explanation for beneficial effects regarding the deletion leading to a variant expression profile in heterozygous carriers of this mutation.
SUBMITTER: Hutter G
PROVIDER: S-EPMC3834523 | biostudies-literature | 2013 Nov
REPOSITORIES: biostudies-literature
Hütter Gero G Blüthgen Christian C Neumann Martin M Reinwald Mark M Nowak Daniel D Klüter Harald H
AIDS research and therapy 20131118 1
<h4>Background</h4>CCR5-delta32 heterozygous individuals are susceptible to HIV-1. However, it is not clear if there is a relevant protective effect against transmission and a beneficial effect in terms of HIV progression which cannot be attributed to CCR5 surface density alone. Therefore we investigated HIV-1 dependency factors (HDF) which might be differently regulated in CCR5 wild type (WT) and CCR5-delta32 heterozygous individuals.<h4>Methods</h4>We examined CD34+ hematopoietic progenitor ce ...[more]