Ontology highlight
ABSTRACT:
SUBMITTER: Shapiro JP
PROVIDER: S-EPMC3835202 | biostudies-literature | 2012 Dec
REPOSITORIES: biostudies-literature
Shapiro John P JP Biswas Sabyasachi S Merchant Anand S AS Satoskar Anjali A Taslim Cenny C Lin Shili S Rovin Brad H BH Sen Chandan K CK Roy Sashwati S Freitas Michael A MA
Journal of proteomics 20120925
This paper describes a simple, highly efficient and robust proteomic workflow for routine liquid-chromatography tandem mass spectrometry analysis of Laser Microdissection Pressure Catapulting (LMPC) isolates. Highly efficient protein recovery was achieved by optimization of a "one-pot" protein extraction and digestion allowing for reproducible proteomic analysis on as few as 500 LMPC isolated cells. The method was combined with label-free spectral count quantitation to characterize proteomic dif ...[more]