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A fluorogenic probe for ?-galactosidase activity imaging in living cells.

ABSTRACT: A cell permeable fluorescence turn-on probe, AcGQCy7, was developed to image ?-galactosidase activity in living cells. Once internalized by ?-galactosidase-expressing cells, the probe was hydrolyzed into a highly fluorescent molecule, and the fluorescent signal was retained in mitochondria for several days. This resulted in a long-lasting and strong ?-galactosidase-dependent intracellular fluorescent signal with little background fluorescence in the culture media.

SUBMITTER: Han J

PROVIDER: S-EPMC3836597 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature

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A fluorogenic probe for β-galactosidase activity imaging in living cells.

Han Junyan J Han Myung Shin MS Tung Ching-Hsuan CH

Molecular bioSystems 20130919 12

A cell permeable fluorescence turn-on probe, AcGQCy7, was developed to image β-galactosidase activity in living cells. Once internalized by β-galactosidase-expressing cells, the probe was hydrolyzed into a highly fluorescent molecule, and the fluorescent signal was retained in mitochondria for several days. This resulted in a long-lasting and strong β-galactosidase-dependent intracellular fluorescent signal with little background fluorescence in the culture media. ...[more]

PMID: 24056749

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