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Rapid and effective removal of perfluorooctanoic acid from proteomics samples.

ABSTRACT: We recently demonstrated that perfluorooctanoic acid (PFOA), a volatile surfactant, is as effective as sodium dodecyl sulfate at solubilizing the membrane proteins. PFOA can be removed by repeated evaporation prior to mass spectrometry analysis. However, the removal of PFOA by evaporation is a lengthy process that takes approximately 6 h. Toward the goal of decreasing the length of time required to remove PFOA from protein digests, we tested the efficiency of PFOA removal and subsequent peptide recovery using strong cation exchange (SCX) chromatography, hydrophilic interaction chromatography (HILIC), fluorous solid phase extraction (FSPE), and anion exchange (ANX) chromatography. We found that all these chromatographic techniques except ANX chromatography remove PFOA thoroughly from protein digest. Peptide recovery rates from the SCX chromatography varied widely; nonacidic peptides were recovered at a rate of up to 95%, while acidic peptides were recovered at a rate of less than 10%. On the other hand, acidic peptides were recovered well from HILIC, while peptides whose pIs are greater than 6 were recovered poorly. Peptide recovery using FSPE was considerably lower, less than 10% for most of the peptides. These results indicate that the SCX and HILIC chromatography provide a more rapid alternative to the evaporation method for applications in which recovery of entire set of peptides is not required.

SUBMITTER: Rao Kadiyala CS

PROVIDER: S-EPMC3840423 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature

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Rapid and effective removal of perfluorooctanoic acid from proteomics samples.

Rao Kadiyala Chandra Sekhar CS Mullangi Vennela V Zhou Xiang X Vukoti Krishna M KM Miyagi Masaru M

Proteomics 20120801 14

We recently demonstrated that perfluorooctanoic acid (PFOA), a volatile surfactant, is as effective as sodium dodecyl sulfate at solubilizing the membrane proteins. PFOA can be removed by repeated evaporation prior to mass spectrometry analysis. However, the removal of PFOA by evaporation is a lengthy process that takes approximately 6 h. Toward the goal of decreasing the length of time required to remove PFOA from protein digests, we tested the efficiency of PFOA removal and subsequent peptide ...[more]

PMID: 22685033

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Project description:Here, we propose a low-cost, sustainable, and viable adsorbent (pine tree-derived biochar) to remove acid dyes such as acid violet 17 (AV), which is used in the silk dyeing industry. As a case study, the AV removal process was demonstrated using synthetic effluent and further as a proof of concept using real dye effluent produced from the Sirumugai textile unit in India. The pine tree-derived biochar was selected for removal of aqueous AV dye in batch and fixed-bed column studies. The adsorbent material was characterized for crystallinity (XRD), surface area (BET), surface morphology and elemental compositions (SEM-EDX), thermal stability (TGA), weight loss (DGA), and functional groups (FTIR). Batch sorption studies were performed to evaluate (i) adsorption at various pH values (at pH 2 to 7), (ii) isotherms (at 10, 25, and 35 °C) to assess the temperature effect on the sorption efficiency, and (iii) kinetics to reveal the effect of time, adsorbent dose, and initial concentration on the reaction rate. After systematic evaluation, 2 g/L biochar, 25 mg/L AV, pH 3, 40 °C, and 40 and 360 min in a completely mixed batch study resulted in 50 and 90% dye removal, respectively. The isoelectric point at pH 3.7 ± 0.2 results in maximum dye removal, therefore suggesting that monitoring the ratio of different effluent (acid/wash/dye) can improve the colorant removal efficiency. The Langmuir isotherm best fits with the sorption of AV to biochar, provided a maximal dye uptake of 29 mg/g at 40 °C, showing that adsorption was endothermic. Fixed-bed studies were conducted at room temperature with an initial dye concentration of 25 and 50 mg/L. The glass columns were packed with biochar (bed depth 20 cm, pore volume = 14 mL) at an initial pH of 5.0 and a 10 mL/min flow rate for 120 min. Finally, the regeneration of the adsorbent was achieved using desorption studies conducted under the proposed experimental conditions resulted in 90-93% removal of AV even after five cycles of regeneration.

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Rapid and effective removal of perfluorooctanoic acid from proteomics samples.

Publications

Rapid and effective removal of perfluorooctanoic acid from proteomics samples.

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