Antigenic analysis of monoclonal antibodies against different epitopes of ?B protein of avian reovirus.

ABSTRACT:

Background

Avian reovirus (ARV) causes arthritis, tenosynovitis, runting-stunting syndrome (RSS), malabsorption syndrome (MAS) and immunosuppression in chickens. ?B is one of the major structural proteins of ARV, which is able to induce group-specific antibodies against the virus.

Methods and results

The present study described the identification of two linear B-cell epitopes in ARV ?B through expressing a set of partially overlapping and consecutive truncated peptides spanning ?B screened with two monoclonal antibodies (mAbs) 1F4 and 1H3-1.The data indicated that (21)KTPACW(26) (epitope A) and (32)WDTVTFH(38) (epitope B) were minimal determinants of the linear B cell epitopes. Antibodies present in the serum of ARV-positive chickens recognized the minimal linear epitopes in Western blot analyses. By sequence alignment analysis, we determined that the epitopes A and B were not conserved among ARV, duck reovirus (DRV) and turkey reovirus (TRV) strains. Western blot assays, confirmed that epitopes A and B were ARV-specific epitopes, and they could not react with the corresponding peptides of DRV and TRV.

Conclusions and significance

We identified (21)KTPACW(26) and (32)WDTVTFH(38) as ?B -specific epitopes recognized by mAbs 1F4 and 1H3-1, respectively. The results in this study may have potential applications in development of diagnostic techniques and epitope-based marker vaccines against ARV groups.

SUBMITTER: Yin CH 

PROVIDER: S-EPMC3842295 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature