Ontology highlight
ABSTRACT:
SUBMITTER: Rems L
PROVIDER: S-EPMC3843160 | biostudies-literature | 2013 Nov
REPOSITORIES: biostudies-literature
Rems Lea L Ušaj Marko M Kandušer Maša M Reberšek Matej M Miklavčič Damijan D Pucihar Gorazd G
Scientific reports 20131129
Electrofusion is an efficient method for fusing cells using short-duration high-voltage electric pulses. However, electrofusion yields are very low when fusion partner cells differ considerably in their size, since the extent of electroporation (consequently membrane fusogenic state) with conventionally used microsecond pulses depends proportionally on the cell radius. We here propose a new and innovative approach to fuse cells with shorter, nanosecond (ns) pulses. Using numerical calculations w ...[more]