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Expression data from HDFA cells exposed to nanosecond duration electrical pulses

ABSTRACT: It is unclear how nanosecond electrical pulses affect gene expression. We used microarrays to identify how cells respond to this specific type of stress

ORGANISM(S): Homo sapiens

PROVIDER: GSE77906 | GEO | 2016/02/16

SECONDARY ACCESSION(S): PRJNA311994

REPOSITORIES: GEO

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Expression data from HDFA cells exposed to nanosecond duration electrical pulses

Project description:It is unclear how nanosecond electrical pulses affect gene expression. We used microarrays to identify how cells respond to this specific type of stress HDFA cells were exposed to 100, 10 nanosecond duration pulses at 150 kV/cm. Gene expression was analyzed 4 hrs post exposure

2016-02-16 | E-GEOD-77906 | biostudies-arrayexpress

Expression data from U-937 cells exposed to nanosecond duration electrical pulses

Project description:It is unclear how nanosecond electrical pulses affect gene expression. We used microarrays to identify how cells respond to this specific type of stress U-937 cells were exposed to 100, 10 nanosecond duration pulses at 150 kV/cm. Gene expression was analyzed 4 hrs post exposure

2016-02-16 | E-GEOD-77908 | biostudies-arrayexpress

Expression data from U-937 cells exposed to nanosecond duration electrical pulses

Project description:It is unclear how nanosecond electrical pulses affect gene expression. We used microarrays to identify how cells respond to this specific type of stress

2016-02-16 | GSE77908 | GEO

Expression data from Jurkat Clone E-6 cells exposed to nanosecond duration electrical pulses

Project description:It is unclear how nanosecond electrical pulses affect gene expression. We used microarrays to identify how cells respond to this specific type of stress Jurkat cells were exposed to 100, 10 nanosecond duration pulses at 150 kV/cm. Gene expression was analyzed 4 hrs post exposure

2016-02-16 | E-GEOD-77907 | biostudies-arrayexpress

Expression data from Jurkat Clone E-6 cells exposed to nanosecond duration electrical pulses

Project description:It is unclear how nanosecond electrical pulses affect gene expression. We used microarrays to identify how cells respond to this specific type of stress

2016-02-16 | GSE77907 | GEO

Adult human dermal fibroblasts exposed to nanosecond electrical pulses exhibit genetic biomarkers of mechanical stress.

Project description:BackgroundExposure of cells to very short (<1 µs) electric pulses in the megavolt/meter range have been shown to cause a multitude of effects, both physical and molecular in nature. Physically, nanosecond electrical pulses (nsEP) can cause disruption of the plasma membrane, cellular swelling, shrinking and blebbing. Molecularly, nsEP have been shown to activate signaling pathways, produce oxidative stress, stimulate hormone secretion and induce both apoptotic and necrotic death. We hypothesize that studying the genetic response of primary human dermal fibroblasts exposed to nsEP, will gain insight into the molecular mechanism(s) either activated directly by nsEP, or indirectly through electrophysiology interactions.MethodsMicroarray analysis in conjunction with quantitative real time polymerase chain reaction (qRT-PCR) was used to screen and validate genes selectively upregulated in response to nsEP exposure.ResultsExpression profiles of 486 genes were found to be significantly changed by nsEP exposure. 50% of the top 20 responding genes coded for proteins located in two distinct cellular locations, the plasma membrane and the nucleus. Further analysis of five of the top 20 upregulated genes indicated that the HDFa cells' response to nsEP exposure included many elements of a mechanical stress response.ConclusionsWe found that several genes, some of which are mechanosensitive, were selectively upregulated due to nsEP exposure. This genetic response appears to be a primary response to the stimuli and not a secondary response to cellular swelling.General significanceThis work provides strong evidence that cells exposed to nsEP interpret the insult as a mechanical stress.

| S-EPMC5614618 | biostudies-literature

Gene expression induced by Nanosecond pulsed electric fields

Project description:We use a human whole genome microarray to analyze the effects of nanosecond pulsed electric fields on Jurkat cells with the focus on early response genes to DNA damage. Keywords: nanosecond pulsed electric fields, jurkat cells, DNA damage

2006-02-01 | GSE4106 | GEO

Homo sapiens

Project description:Expression data from HDFA cells exposed to nanosecond duration electrical pulses

| PRJNA311994 | ENA

Homo sapiens

Project description:Expression data from U-937 cells exposed to nanosecond duration electrical pulses

| PRJNA311996 | ENA

Ion transport into cells exposed to monopolar and bipolar nanosecond pulses.

Project description:Experiments with CHO cells exposed to 60 and 300 ns pulsed electric fields with amplitudes in the range from several kV/cm to tens of kV/cm showed a decrease of the uptake of calcium ions by more than an order of magnitude when, immediately after a first pulse, a second one of opposite polarity was applied. This effect is assumed to be due to the reversal of the electrophoretic transport of ions through the electroporated membrane during the second phase of the bipolar pulse. This assumption, however, is only valid if electrophoresis is the dominant transport mechanism, rather than diffusion. Comparison of calculated calcium ion currents with experimental results showed that for nanosecond pulses, electrophoresis is at least as important as diffusion. By delaying the second pulse with respect to the first one, the effect of reverse electrophoresis is reduced. Consequently, separating nanosecond pulses of opposite polarity by up to approximately hundred microseconds allows us to vary the uptake of ions from very small values to those obtained with two pulses of the same polarity. The measured calcium ion uptake obtained with bipolar pulses also allowed us to determine the membrane pore recovery time. The calculated recovery time constants are on the order of 10 μs.

| S-EPMC4345159 | biostudies-literature

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