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Absence of detectable arsenate in DNA from arsenate-grown GFAJ-1 cells.

ABSTRACT: A strain of Halomonas bacteria, GFAJ-1, has been claimed to be able to use arsenate as a nutrient when phosphate is limiting and to specifically incorporate arsenic into its DNA in place of phosphorus. However, we have found that arsenate does not contribute to growth of GFAJ-1 when phosphate is limiting and that DNA purified from cells grown with limiting phosphate and abundant arsenate does not exhibit the spontaneous hydrolysis expected of arsenate ester bonds. Furthermore, mass spectrometry showed that this DNA contains only trace amounts of free arsenate and no detectable covalently bound arsenate.

SUBMITTER: Reaves ML

PROVIDER: S-EPMC3845625 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature

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Absence of detectable arsenate in DNA from arsenate-grown GFAJ-1 cells.

Reaves Marshall Louis ML Sinha Sunita S Rabinowitz Joshua D JD Kruglyak Leonid L Redfield Rosemary J RJ

Science (New York, N.Y.) 20120708 6093

A strain of Halomonas bacteria, GFAJ-1, has been claimed to be able to use arsenate as a nutrient when phosphate is limiting and to specifically incorporate arsenic into its DNA in place of phosphorus. However, we have found that arsenate does not contribute to growth of GFAJ-1 when phosphate is limiting and that DNA purified from cells grown with limiting phosphate and abundant arsenate does not exhibit the spontaneous hydrolysis expected of arsenate ester bonds. Furthermore, mass spectrometry ...[more]

PMID: 22773140

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