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The relationship between membrane potential and calcium dynamics in glucose-stimulated beta cell syncytium in acute mouse pancreas tissue slices.

ABSTRACT: Oscillatory electrical activity is regarded as a hallmark of the pancreatic beta cell glucose-dependent excitability pattern. Electrophysiologically recorded membrane potential oscillations in beta cells are associated with in-phase oscillatory cytosolic calcium activity ([Ca(2+)]i) measured with fluorescent probes. Recent high spatial and temporal resolution confocal imaging revealed that glucose stimulation of beta cells in intact islets within acute tissue slices produces a [Ca(2+)]i change with initial transient phase followed by a plateau phase with highly synchronized [Ca(2+)]i oscillations. Here, we aimed to correlate the plateau [Ca(2+)]i oscillations with the oscillations of membrane potential using patch-clamp and for the first time high resolution voltage-sensitive dye based confocal imaging. Our results demonstrated that the glucose-evoked membrane potential oscillations spread over the islet in a wave-like manner, their durations and wave velocities being comparable to the ones for [Ca(2+)]i oscillations and waves. High temporal resolution simultaneous records of membrane potential and [Ca(2+)]i confirmed tight but nevertheless limited coupling of the two processes, with membrane depolarization preceding the [Ca(2+)]i increase. The potassium channel blocker tetraethylammonium increased the velocity at which oscillations advanced over the islet by several-fold while, at the same time, emphasized differences in kinetics of the membrane potential and the [Ca(2+)]i. The combination of both imaging techniques provides a powerful tool that will help us attain deeper knowledge of the beta cell network.

SUBMITTER: Dolensek J

PROVIDER: S-EPMC3855743 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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The relationship between membrane potential and calcium dynamics in glucose-stimulated beta cell syncytium in acute mouse pancreas tissue slices.

Dolenšek Jurij J Stožer Andraž A Skelin Klemen Maša M Miller Evan W EW Slak Rupnik Marjan M

PloS one 20131206 12

Oscillatory electrical activity is regarded as a hallmark of the pancreatic beta cell glucose-dependent excitability pattern. Electrophysiologically recorded membrane potential oscillations in beta cells are associated with in-phase oscillatory cytosolic calcium activity ([Ca(2+)]i) measured with fluorescent probes. Recent high spatial and temporal resolution confocal imaging revealed that glucose stimulation of beta cells in intact islets within acute tissue slices produces a [Ca(2+)]i change w ...[more]

PMID: 24324777

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Dataset Information

The relationship between membrane potential and calcium dynamics in glucose-stimulated beta cell syncytium in acute mouse pancreas tissue slices.

Publications

The relationship between membrane potential and calcium dynamics in glucose-stimulated beta cell syncytium in acute mouse pancreas tissue slices.

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