Ontology highlight
ABSTRACT:
SUBMITTER: Sanghvi JC
PROVIDER: S-EPMC3856890 | biostudies-literature | 2013 Dec
REPOSITORIES: biostudies-literature
Sanghvi Jayodita C JC Regot Sergi S Carrasco Silvia S Karr Jonathan R JR Gutschow Miriam V MV Bolival Benjamin B Covert Markus W MW
Nature methods 20131103 12
To test the promise of whole-cell modeling to facilitate scientific inquiry, we compared growth rates simulated in a whole-cell model with experimental measurements for all viable single-gene disruption Mycoplasma genitalium strains. Discrepancies between simulations and experiments led to predictions about kinetic parameters of specific enzymes that we subsequently validated. These findings represent, to our knowledge, the first application of whole-cell modeling to accelerate biological discov ...[more]