Ontology highlight
ABSTRACT:
SUBMITTER: Otani J
PROVIDER: S-EPMC3858372 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Otani Junji J Kimura Hironobu H Sharif Jafar J Endo Takaho A TA Mishima Yuichi Y Kawakami Toru T Koseki Haruhiko H Shirakawa Masahiro M Suetake Isao I Tajima Shoji S
PloS one 20131210 12
Hydroxymethylcytosine in the genome is reported to be an intermediate of demethylation. In the present study, we demonstrated that maintenance methyltransferase Dnmt1 scarcely catalyzed hemi-hydroxymethylated DNA and that the hemi-hydroxymethylated DNA was not selectively recognized by the SRA domain of Uhrf1, indicating that hydroxymethylcytosine is diluted in a replication-dependent manner. A high level of 5-hydroxymethylcytosine in mouse embryonic stem cells was produced from the methylcytosi ...[more]