Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia.
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ABSTRACT:
SUBMITTER: Su S
PROVIDER: S-EPMC3860264 | biostudies-literature | 2013 Nov
REPOSITORIES: biostudies-literature
Publications
Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia.
Su Steven S Phua Siew Cheng SC DeRose Robert R Chiba Shuhei S Narita Keishi K Kalugin Peter N PN Katada Toshiaki T Kontani Kenji K Takeda Sen S Inoue Takanari T
Nature methods 20130922 11
Visualization of signal transduction in live primary cilia constitutes a technical challenge owing to the organelle's submicrometer dimensions and close proximity to the cell body. Using a genetically encoded calcium indicator targeted to primary cilia, we visualized calcium signaling in cilia of mouse fibroblasts and kidney cells upon chemical or mechanical stimulation with high specificity, high sensitivity and wide dynamic range. ...[more]
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