Unknown

Dataset Information

0

A near-infrared genetically encoded calcium indicator for in vivo imaging.


ABSTRACT: While calcium imaging has become a mainstay of modern neuroscience, the spectral properties of current fluorescent calcium indicators limit deep-tissue imaging as well as simultaneous use with other probes. Using two monomeric near-infrared (NIR) fluorescent proteins (FPs), we engineered an NIR Förster resonance energy transfer (FRET)-based genetically encoded calcium indicator (iGECI). iGECI exhibits high levels of brightness and photostability and an increase up to 600% in the fluorescence response to calcium. In dissociated neurons, iGECI detects spontaneous neuronal activity and electrically and optogenetically induced firing. We validated the performance of iGECI up to a depth of almost 400 µm in acute brain slices using one-photon light-sheet imaging. Applying hybrid photoacoustic and fluorescence microscopy, we simultaneously monitored neuronal and hemodynamic activities in the mouse brain through an intact skull, with resolutions of ~3 μm (lateral) and ~25-50 μm (axial). Using two-photon imaging, we detected evoked and spontaneous neuronal activity in the mouse visual cortex, with fluorescence changes of up to 25%. iGECI allows biosensors and optogenetic actuators to be multiplexed without spectral crosstalk.

SUBMITTER: Shemetov AA 

PROVIDER: S-EPMC7956128 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC6393164 | biostudies-literature
| S-EPMC7723245 | biostudies-literature
| S-EPMC6678319 | biostudies-literature
| S-EPMC10370049 | biostudies-literature
| S-EPMC9468011 | biostudies-literature
| S-EPMC3860264 | biostudies-literature
| S-EPMC7555670 | biostudies-literature
| S-EPMC6722131 | biostudies-literature
| S-EPMC3593221 | biostudies-literature
| S-EPMC8564460 | biostudies-literature