Genome-wide mutagenesis of influenza virus reveals unique plasticity of the hemagglutinin and NS1 proteins.
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ABSTRACT:
SUBMITTER: Heaton NS
PROVIDER: S-EPMC3864309 | biostudies-literature | 2013 Dec
REPOSITORIES: biostudies-literature
Publications
Genome-wide mutagenesis of influenza virus reveals unique plasticity of the hemagglutinin and NS1 proteins.
Heaton Nicholas S NS Sachs David D Chen Chi-Jene CJ Hai Rong R Palese Peter P
Proceedings of the National Academy of Sciences of the United States of America 20131125 50
The molecular basis for the diversity across influenza strains is poorly understood. To gain insight into this question, we mutagenized the viral genome and sequenced recoverable viruses. Only two small regions in the genome were enriched for insertions, the hemagglutinin head and the immune-modulatory nonstructural protein 1. These proteins play a major role in host adaptation, and thus need to be able to evolve rapidly. We propose a model in which certain influenza A virus proteins (or protein ...[more]
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