Genome-wide transposon mutagenesis of paramyxoviruses reveals constraints on genomic plasticity.
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ABSTRACT:
SUBMITTER: Ikegame S
PROVIDER: S-EPMC7577504 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
Publications
Genome-wide transposon mutagenesis of paramyxoviruses reveals constraints on genomic plasticity.
Ikegame Satoshi S Beaty Shannon M SM Stevens Christian C Won Taylor T Park Arnold A Sachs David D Hong Patrick P Lee Benhur B Thibault Patricia A PA
PLoS pathogens 20201009 10
The antigenic and genomic stability of paramyxoviruses remains a mystery. Here, we evaluate the genetic plasticity of Sendai virus (SeV) and mumps virus (MuV), sialic acid-using paramyxoviruses that infect mammals from two Paramyxoviridae subfamilies (Orthoparamyxovirinae and Rubulavirinae). We performed saturating whole-genome transposon insertional mutagenesis, and identified important commonalities: disordered regions in the N and P genes near the 3' genomic end were more tolerant to insertio ...[more]
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