Ontology highlight
ABSTRACT:
SUBMITTER: Porchetta A
PROVIDER: S-EPMC3866043 | biostudies-literature | 2012 Dec
REPOSITORIES: biostudies-literature
Porchetta Alessandro A Vallée-Bélisle Alexis A Plaxco Kevin W KW Ricci Francesco F
Journal of the American Chemical Society 20121213 51
Here we demonstrate multiple, complementary approaches by which to tune, extend, or narrow the dynamic range of aptamer-based sensors. Specifically, we employ both distal-site mutations and allosteric control to tune the affinity and dynamic range of a fluorescent aptamer beacon. We show that allosteric control, achieved by using a set of easily designed oligonucleotide inhibitors that competes against the folding of the aptamer, allows rational fine-tuning of the affinity of our model aptamer a ...[more]