Inactivation of metabolic genes causes short- and long-range dys-regulation in Escherichia coli metabolic network.

ABSTRACT: The metabolic network in E. coli can be severely affected by the inactivation of metabolic genes that are required to catabolize a nutrient (D-galactose). We hypothesized that the resulting accumulation of small molecules can yield local as well as systemic effects on the metabolic network. Analysis of metabolomics data in wild-type and D-galactose non-utilizing mutants, galT, galU and galE, reveal the large metabolic differences between the wild-type and the mutants when the strains were grown in D-galactose. Network mapping suggested that the enzymatic defects affected the metabolic modules located both at short- and long-ranges from the D-galactose metabolic module. These modules suggested alterations in glutathione, energy, nucleotide and lipid metabolism and disturbed carbon to nitrog

SUBMITTER: Barupal DK 

PROVIDER: S-EPMC3868466 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature