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Detergent-free mass spectrometry of membrane protein complexes.

ABSTRACT: We developed a method that allows release of intact membrane protein complexes from amphipols, bicelles and nanodiscs in the gas phase for observation by mass spectrometry (MS). Current methods involve release of membrane protein complexes from detergent micelles, which reveals subunit composition and lipid binding. We demonstrated that oligomeric complexes or proteins requiring defined lipid environments are stabilized to a greater extent in the absence of detergent.

SUBMITTER: Hopper JT

PROVIDER: S-EPMC3868940 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature

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Detergent-free mass spectrometry of membrane protein complexes.

Hopper Jonathan T S JT Yu Yvonne Ting-Chun YT Li Dianfan D Raymond Alison A Bostock Mark M Liko Idlir I Mikhailov Victor V Laganowsky Arthur A Benesch Justin L P JL Caffrey Martin M Nietlispach Daniel D Robinson Carol V CV

Nature methods 20131013 12

We developed a method that allows release of intact membrane protein complexes from amphipols, bicelles and nanodiscs in the gas phase for observation by mass spectrometry (MS). Current methods involve release of membrane protein complexes from detergent micelles, which reveals subunit composition and lipid binding. We demonstrated that oligomeric complexes or proteins requiring defined lipid environments are stabilized to a greater extent in the absence of detergent. ...[more]

PMID: 24122040

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