Ontology highlight
ABSTRACT:
SUBMITTER: Laganowsky A
PROVIDER: S-EPMC4058633 | biostudies-literature | 2013 Apr
REPOSITORIES: biostudies-literature
Nature protocols 20130307 4
Mass spectrometry (MS) of intact soluble protein complexes has emerged as a powerful technique to study the stoichiometry, structure-function and dynamics of protein assemblies. Recent developments have extended this technique to the study of membrane protein complexes, where it has already revealed subunit stoichiometries and specific phospholipid interactions. Here we describe a protocol for MS of membrane protein complexes. The protocol begins with the preparation of the membrane protein comp ...[more]