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Structural basis of myosin V Rab GTPase-dependent cargo recognition.


ABSTRACT: Specific recognition of the cargo that molecular motors transport or tether to cytoskeleton tracks allows them to perform precise cellular functions at particular times and positions in cells. However, very little is known about how evolution has favored conservation of functions for some isoforms, while also allowing for the generation of new recognition sites and specialized cellular functions. Here we present several crystal structures of the myosin Va or the myosin Vb globular tail domain (GTD) that gives insights into how the motor is linked to the recycling membrane compartments via Rab11 or to the melanosome membrane via recognition of the melanophilin adaptor that binds to Rab27a. The structures illustrate how the Rab11-binding site has been conserved during evolution and how divergence at another site of the GTD allows more specific interactions such as the specific recognition of melanophilin by the myosin Va isoform. With atomic structural insights, these structures also show how either the partner or the GTD structural plasticity upon association is critical for selective recruitment of the motor.

SUBMITTER: Pylypenko O 

PROVIDER: S-EPMC3870677 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature

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Structural basis of myosin V Rab GTPase-dependent cargo recognition.

Pylypenko Olena O   Attanda Wikayatou W   Gauquelin Charles C   Lahmani Marion M   Coulibaly Doudouh D   Baron Bruno B   Hoos Sylviane S   Titus Margaret A MA   England Patrick P   Houdusse Anne M AM  

Proceedings of the National Academy of Sciences of the United States of America 20131118 51


Specific recognition of the cargo that molecular motors transport or tether to cytoskeleton tracks allows them to perform precise cellular functions at particular times and positions in cells. However, very little is known about how evolution has favored conservation of functions for some isoforms, while also allowing for the generation of new recognition sites and specialized cellular functions. Here we present several crystal structures of the myosin Va or the myosin Vb globular tail domain (G  ...[more]

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