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Photobleaching lifetimes of cyanine fluorophores used for single-molecule Forster resonance energy transfer in the presence of various photoprotection systems.

ABSTRACT: Lengthening smFRET lifetimes: We investigated various photoprotection system combinations to find the combination that optimally extended the photobleach lifetime of a Cy3/Cy5 smFRET pair attached to a DNA hairpin in a single-molecule environment. We found that the glucose/glucose oxygen-scavenging solution in combination with redox-based photostabilization solutions yielded the longest average photobleaching lifetimes.

SUBMITTER: Cooper D

PROVIDER: S-EPMC3871170 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature

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Photobleaching lifetimes of cyanine fluorophores used for single-molecule Förster resonance energy transfer in the presence of various photoprotection systems.

Cooper David D Uhm Heui H Tauzin Lawrence J LJ Poddar Nitesh N Landes Christy F CF

Chembiochem : a European journal of chemical biology 20130603 9

Lengthening smFRET lifetimes: We investigated various photoprotection system combinations to find the combination that optimally extended the photobleach lifetime of a Cy3/Cy5 smFRET pair attached to a DNA hairpin in a single-molecule environment. We found that the glucose/glucose oxygen-scavenging solution in combination with redox-based photostabilization solutions yielded the longest average photobleaching lifetimes. ...[more]

PMID: 23733413

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