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Synthesis of nitric oxide probes with fluorescence lifetime sensitivity.

ABSTRACT: We present the rationale, synthesis and evaluation of the first activatable fluorescent probe that utilizes fluorescence lifetime change for detection of nitric oxide. The new probe DAP-LT1 features a near-infrared polymethine skeleton with a diaminobenzene functionality incorporated into the meso-position. The probe is partially quenched, and upon reaction with nitric oxide shows an increase in the fluorescence lifetime from 1.08 ns to 1.24 ns.

SUBMITTER: Zhegalova NG

PROVIDER: S-EPMC3872128 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature

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Synthesis of nitric oxide probes with fluorescence lifetime sensitivity.

Zhegalova Natalia G NG Gonzales Garrett G Berezin Mikhail Y MY

Organic & biomolecular chemistry 20131028 47

We present the rationale, synthesis and evaluation of the first activatable fluorescent probe that utilizes fluorescence lifetime change for detection of nitric oxide. The new probe DAP-LT1 features a near-infrared polymethine skeleton with a diaminobenzene functionality incorporated into the meso-position. The probe is partially quenched, and upon reaction with nitric oxide shows an increase in the fluorescence lifetime from 1.08 ns to 1.24 ns. ...[more]

PMID: 24166035

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