Ontology highlight
ABSTRACT:
SUBMITTER: Paetzold B
PROVIDER: S-EPMC3874219 | biostudies-literature | 2013 Dec
REPOSITORIES: biostudies-literature
Paetzold Bernhard B Carolis Carlo C Ferrar Tony T Serrano Luis L Lluch-Senar Maria M
ACS synthetic biology 20131106 12
Modern cloning methods are independent from restriction enzyme recognition sites. However, nearly all current cloning methods still require the introduction of overlaps by PCR, which can introduce undesired mutations. Here, we investigated whether overlaps needed for DNA assembly can be synthesized in situ and we tested if the de novo synthesis of sequences can be simultaneously combined with the assembly of larger double-stranded DNA fragments. We showed in a set of 44 cloning experiments that ...[more]