Ontology highlight
ABSTRACT:
SUBMITTER: Haas BJ
PROVIDER: S-EPMC3875132 | biostudies-literature | 2013 Aug
REPOSITORIES: biostudies-literature
Haas Brian J BJ Papanicolaou Alexie A Yassour Moran M Grabherr Manfred M Blood Philip D PD Bowden Joshua J Couger Matthew Brian MB Eccles David D Li Bo B Lieber Matthias M MacManes Matthew D MD Ott Michael M Orvis Joshua J Pochet Nathalie N Strozzi Francesco F Weeks Nathan N Westerman Rick R William Thomas T Dewey Colin N CN Henschel Robert R LeDuc Richard D RD Friedman Nir N Regev Aviv A
Nature protocols 20130711 8
De novo assembly of RNA-seq data enables researchers to study transcriptomes without the need for a genome sequence; this approach can be usefully applied, for instance, in research on 'non-model organisms' of ecological and evolutionary importance, cancer samples or the microbiome. In this protocol we describe the use of the Trinity platform for de novo transcriptome assembly from RNA-seq data in non-model organisms. We also present Trinity-supported companion utilities for downstream applicati ...[more]