Evaluation of porB PCR-amplicon restriction endonuclease analysis as a method to determine porB variable-region sequences in nonserotypeable meningococci.
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ABSTRACT:
SUBMITTER: Dyet KH
PROVIDER: S-EPMC387555 | biostudies-literature | 2004 Apr
REPOSITORIES: biostudies-literature
Publications
Evaluation of porB PCR-amplicon restriction endonuclease analysis as a method to determine porB variable-region sequences in nonserotypeable meningococci.
Dyet Kristin H KH Simmonds Robin S RS Martin Diana R DR
Journal of clinical microbiology 20040401 4
porB PCR-amplicon restriction endonuclease analysis is a rapid, simple method developed to assess porB variation in nonserotypeable meningococci isolated during New Zealand's epidemic of meningococcal disease. Most nonserotypeable meningococci isolated between 1990 and 1999 inclusively either were type 4 (40.5%) or contained the porB variable region 1 (VR1)-19, VR2-D, VR3-7, and VR4-14a sequences (45.1%). ...[more]
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