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Simple Algorithm for Identification of Bordetella pertussis Pertactin Gene Variants.

ABSTRACT: Studies performed in several countries have demonstrated the recent emergence and subsequent dominance of circulating Bordetella pertussis strains harboring pertactin and pertussis toxin variants not included in pertussis vaccines. Determination of the pertactin gene variants is commonly performed using a time-consuming and expensive sequence analysis. We developed a simple and reliable pertactin typing algorithm suitable for large-scale screening. The assay correctly identified all pertactin alleles in representative strains. The typing of 231 clinical strains of B. pertussis routinely isolated in Belgium showed that this algorithm was adequate to identify less-frequent prn types like prn9 and prn11.

SUBMITTER: Muyldermans G

PROVIDER: S-EPMC387558 | biostudies-literature | 2004 Apr

REPOSITORIES: biostudies-literature

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Simple Algorithm for Identification of Bordetella pertussis Pertactin Gene Variants.

Muyldermans Gaëtan G Piérard Denis D Hoebrekx Nathalie N Advani Reza R Van Amersfoorth Shirley S De Schutter Iris I Soetens Oriane O Eeckhout Leo L Malfroot Anne A Lauwers Sabine S

Journal of clinical microbiology 20040401 4

Studies performed in several countries have demonstrated the recent emergence and subsequent dominance of circulating Bordetella pertussis strains harboring pertactin and pertussis toxin variants not included in pertussis vaccines. Determination of the pertactin gene variants is commonly performed using a time-consuming and expensive sequence analysis. We developed a simple and reliable pertactin typing algorithm suitable for large-scale screening. The assay correctly identified all pertactin al ...[more]

PMID: 15071014

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