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High-purity prostate circulating tumor cell isolation by a polymer nanofiber-embedded microchip for whole exome sequencing.


ABSTRACT: Handpick single cancer cells: a modified NanoVelcro Chip is coupled with ArcturusXT laser capture microdissection (LCM) technology to enable the detection and isolation of single circulating tumor cells (CTCs) from patients with prostate cancer (PC). This new approach paves the way for conducting next-generation sequencing (NGS) on single CTCs.

SUBMITTER: Zhao L 

PROVIDER: S-EPMC3875622 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature

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High-purity prostate circulating tumor cell isolation by a polymer nanofiber-embedded microchip for whole exome sequencing.

Zhao Libo L   Lu Yi-Tsung YT   Li Fuqiang F   Wu Kui K   Hou Shuang S   Yu Juehua J   Shen Qinglin Q   Wu Dongxia D   Song Min M   OuYang Wei-Han WH   Luo Zheng Z   Lee Tom T   Fang Xiaohong X   Shao Chen C   Xu Xun X   Garcia Mitch A MA   Chung Leland W K LW   Rettig Matthew M   Tseng Hsian-Rong HR   Posadas Edwin M EM  

Advanced materials (Deerfield Beach, Fla.) 20130326 21


Handpick single cancer cells: a modified NanoVelcro Chip is coupled with ArcturusXT laser capture microdissection (LCM) technology to enable the detection and isolation of single circulating tumor cells (CTCs) from patients with prostate cancer (PC). This new approach paves the way for conducting next-generation sequencing (NGS) on single CTCs. ...[more]

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