Genotyping of enteric adenoviruses by using single-stranded conformation polymorphism analysis and heteroduplex mobility assay.
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ABSTRACT:
SUBMITTER: Soares CC
PROVIDER: S-EPMC387600 | biostudies-literature | 2004 Apr
REPOSITORIES: biostudies-literature
Publications
Genotyping of enteric adenoviruses by using single-stranded conformation polymorphism analysis and heteroduplex mobility assay.
Soares Caroline C CC Volotão Eduardo M EM Albuquerque Maria Carolina M MC Nozawa Carlos M CM Linhares Rosa Elisa C RE Volokhov Dmitriy D Chizhikov Vladimir V Lu Xiaoyan X Erdman Dean D Santos Norma N
Journal of clinical microbiology 20040401 4
Single-stranded conformation polymorphism (SSCP) analysis and heteroduplex mobility assays (HMAs) were used to identify and genotype enteric adenoviruses (EAd). The results were compared to those of restriction endonuclease assays, species-specific PCRs, and direct nucleotide sequence analyses. Of the 31 stool samples tested, 15 isolates were identified as EAd and 7 were identified as nonenteric Ad by all methods. An agreement of 100% was found between the SSCP and HMA results. ...[more]
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